Our laboratory has much experience in the production of polymers to influence cellular adhesion, for example dextran, PLL-g-PEG polymer and nanoparticle coatings on biosensor surfaces. We apply flagellin based biomimetic coatings as well to alter the adhesivity of the cells.
Collaboration partners: University of Pannonia, Budapest University of Technology and Economics.


Representative AFM image of titanate nanotube (TNT) coating on OWLS chip.

Nanoparticle clusters
Schematic illustration of nanoparticle cluster buildup from oppositely charged nanoparticles on OWLS chip.

nA–adsorbed mass curves recorded from carboxymetilated dextran (CMD) grafting experiments at different conditions.
A: aminosilylated, E: epoxysilylated surfaces (by the arrows, G refers to the grafting, W to the 
washing phase).
The change in the structure of the CMD layer on the surface is schematically drawn.

Preparation of flagellin-based molecular coatings. Flagellar filaments are removed from the bodies
of bacteria by vortexing and purified in several 
centrifugation steps. Next, filaments are heat-treated
to obtain monomer flagellin units. On the hydrophobic surface flagellins form an oriented 
monolayer.(a) Wild-type flagellin. (b) RGD (Arginylglycylaspartic acid) displaying
flagellin variants. The displayed peptide sequences are also shown.


Schematic representation of the Poly(allylamine) hydrochloride (PAH)–monomeric flagellin composite film buildup.
The inset shows a filament starting PAH induced flagellin nucleation and subsequent polymerization.


Relevant publications: