The Nanobiosensorics “Lendület” Group started its activity in July, 2012. The research profile is the development and application of label-free optical biosensors, the mathematical modeling of the relevant biological and biophysical processes. The group made important steps both in the development of the research infrastructure and both in creating personal and collaborative network. The established - in Hungary not yet conducted - research lines are promising. In several topics (instrumental development, monitoring microvesicles, cell adhesion, flagellin based functional layers, modeling) the laboratory could achieve strategic results, which were published in important international journals (Analytical Chemisty, Biosensors and Bioelectronics, Scientific Reports, Sensors and Actuators B, International Journal of Molecular Sciences, Langmuir, Colloids and Interfaces B, Plos One, Applied Physics Letters etc.), or are under submission.
Our group has established a cell culture laboratory, making possible to launch a new and promising research line, the “label-free biosensorics of living cells”. We have installed an EPIC multichannel biosensor prototype and an M4 Holomonitor. It is important to note that both instruments are quite unique both nationally and internationally. We are among the first who can develop and apply these modern techniques. Not less importantly, the group had a collaboration with the Swiss startup, Creoptix AG on high resolution label-free optical sensing. Our collaboration partners are scientists from famous Hungarian universities, such as Semmelweis University, Eötvös Lóránd University, Budapest University of Technology and Economics, and University of Pannonia.
It is worth mentioning that we were the first who could measure the adhesion properties of microvesicles secreted by living cells and the surface adhesion kinetics of human monocytes using label-free optical biochips. Considering cancer cell adhesion and spreading the group first established a methodology to determine the integrin receptor-RGD ligand affinity constant in living cells without applying any labelling. Our results on measuring the structural order of nanometer scale biological layers (proteins, lipid bilayers) are similarly important. These results can find further applications in biotechnology and medical diagnostics.